FastQCFastQC Report
Sex 16 fev 2018
Cerebro-femea_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCerebro-femea_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17119481
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6891794.025700311826042No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC812560.4746405571524043TruSeq Adapter, Index 3 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT676430.3951229596271055No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN539870.3153541862630065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA112550.054.312491
ATCGGAA119450.051.557652
TCGGAAG125950.048.9228823
TATGCCG128000.047.6514646
CTCGTAT128550.046.34397542
CGTATGC132400.046.13601744
CGGAAGA138050.044.6851434
TCGTATG137250.044.17181843
ACTTAGG139900.043.96253632
CTTAGGC140600.043.67425533
GTATGCC146000.041.915845
TTAGGCA149050.041.3423934
CACTTAG149950.040.987631
TCTCGTA146450.040.9601841
AAAAGGG206200.040.50390270
TAGGCAT154700.039.7230535
ATGCCGT155550.039.3709847
CGTCTGA159800.038.60009816
ATCTCGT156700.038.27776740
TCACTTA163500.037.73697330
Produced by FastQC (version 0.11.4)