FastQCFastQC Report
Thu 17 Oct 2019
female_brain.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefemale_brain.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298842
Sequences flagged as poor quality0
Sequence length57-1200
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTACACACTCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTG62482.090736911143681No Hit
GACTACGTACACACTCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAG28900.9670662088996862No Hit
GACTACGTACACACTCTCACCCGGCCCGGACACGGAAAGGATTGACAGAT21540.7207822193667557No Hit
GACTACGTACACACTCTGCCAGTAACATATGCTTGTCTCAAAGATTAAGC19920.6665729716706487No Hit
GACTACGTACACACTCCTCACCCGGCCCGGACACGGAAAGGATTGACAGA17800.5956324746856199No Hit
GACTACGTACACACTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGAT16670.5578198512926563No Hit
GACTACGTACACACTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTC14610.4888871042222981No Hit
GACTACGTACACACTCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAA14410.48219460450672935No Hit
GACTACGTACACACTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCA14200.4751674798053821No Hit
GACTACGTACACACTGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTA13010.43534710649774794No Hit
GACTACGTACACACTGTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACAC12900.43166623165418516No Hit
GACTACGTACACACTCCTGCCAGTAACATATGCTTGTCTCAAAGATTAAG12510.4186158572088261No Hit
GACTACGTACACACTAGCAACTTTAATATACGCTATTGGAGCTGGAATTA11830.3958613581758923No Hit
GACTACGTACACACTGTAACATATGCTTGTCTCAAAGATTAAGCCATGCA10140.33930973557933625No Hit
GACTACGTACACACTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATAT10010.33495961076421654No Hit
GACTACGTACACACTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTG8670.29011986266990586No Hit
GACTACGTACACACTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG8180.2737232383667624No Hit
GACTACGTACACACTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAA7770.2600036139498464No Hit
GACTACGTACACACTCGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCA7300.24427623961825984No Hit
GACTACGTACACACTGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGC6840.22888349027245167No Hit
GACTACGTACACACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATGG6550.21917936568487695No Hit
GACTACGTACACACTGCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT6420.21482924086975727No Hit
GACTACGTACACACTCCCGGCCCGGACACGGAAAGGATTGACAGATTGAT6370.2131561159408651No Hit
GACTACGTACACACTGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGG6320.21148299101197288No Hit
GACTACGTACACACTGCAACTTTAATATACGCTATTGGAGCTGGAATTAC6290.21047911605463757No Hit
GACTACGTACACACTGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTA6280.21014449106885913No Hit
GACTACGTACACACTCGACTTTTACTTCCTCTAGATAGTCAAGTTCGATC6240.20880599112574538No Hit
GACTACGTACACACTCTCGCCTCGGCCGGCGCCTAGCAGCTGACTTAGAA6210.20780211616841007No Hit
GACTACGTACACACTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTG6050.20244811639595506No Hit
GACTACGTACACACTGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATAC6040.2021134914101766No Hit
GACTACGTACACACTGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTA5980.200105741495506No Hit
GACTACGTACACACTCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAA5830.1950863667088294No Hit
GACTACGTACACACTCCAAGATCTGCACCCGCGGCGGCTCCACCCGGGCC5820.194751741723051No Hit
GACTACGTACACACTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT5780.1934132417799372No Hit
GACTACGTACACACTATTGGAATGAGTACACTTTAAATCCTTTAACGAGG5630.18839386699326066No Hit
GACTACGTACACACTCCCGCTTTCACGGTCTGTACTCATACTGAAAATCA5550.18571686710703314No Hit
GACTACGTACACACTAAGAAGTTGGCCGCCGACCGCTCGGGGCCCCGTAA5500.18404374217814096No Hit
GACTACGTACACACTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACT5480.18337449220658408No Hit
GACTACGTACACACTGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACG5160.17266649266167405No Hit
GACTACGTACACACTCCCAGTCAAACTCCCCACCTGCCACTGTCCCCGGA5140.17199724269011718No Hit
GACTACGTACACACTGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAA5130.17166261770433874No Hit
GACTACGTACACACTGTTGCCGGCGACGGCCGGGTATGGGCCCGACGCTC5100.17065874274700343No Hit
GACTACGTACACACTGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG5080.16998949277544656No Hit
GACTACGTACACACTTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATT5040.1686509928323328No Hit
GACTACGTACACACTAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGG4980.16664324291766217No Hit
GACTACGTACACACTGGTAAAGCGAATGACTAGAGGTCTTGGGGCCGAAA4930.16497011798876998No Hit
GACTACGTACACACTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGC4910.1643008680172131No Hit
GACTACGTACACACTGGAATGAGTACACTTTAAATCCTTTAACGAGGATC4870.16296236807409936No Hit
GACTACGTACACACTCTTGTTACGACTTTTTACTTCCTCTAGATAGTCAA4770.159616118216315No Hit
GACTACGTACACACTCTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGT4710.15760836830164435No Hit
GACTACGTACACACTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGA4640.15526599340119526No Hit
GACTACGTACACACTCACCCGGCCCGGACACGGAAAGGATTGACAGATTG4610.15426211844385998No Hit
GACTACGTACACACTCAGATTGATAGCTCTTTCTCGATTCTGTGGGTGGT4600.15392749345808152No Hit
GACTACGTACACACTCGAATGTCTGCCCTATCAACTTTCGATGGTACTTT4600.15392749345808152No Hit
GACTACGTACACACTGGAAACTCTGGTGGAGGTCCGCAGCGGTCCTGACG4580.15325824348652464No Hit
GACTACGTACACACTCTCGTTCATGGGGAATAATTGCAATCCCCGATCCC4480.14991199362874028No Hit
GACTACGTACACACTGTTTGACTGGGGCGGTACACCTGTCAAACGGTAAC4460.1492427436571834No Hit
GACTACGTACACACTGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA4400.14723499374251278No Hit
GACTACGTACACACTGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC4310.14422336887050682No Hit
GACTACGTACACACTGCAAGAGCGCGCGCGACGGTACCCGAAAGATGGTG4300.1438887438847284No Hit
GACTACGTACACACTCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAAC4260.14255024394161464No Hit
GACTACGTACACACTGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTG4230.14154636898427933No Hit
GACTACGTACACACTCTCATACTGAAAATCAAGATCAAGCGAGCTTTTGC4230.14154636898427933No Hit
GACTACGTACACACTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTC4110.13753086915493806No Hit
GACTACGTACACACTGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGA4100.13719624416915963No Hit
GACTACGTACACACTGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAA4090.1368616191833812No Hit
GACTACGTACACACTCCGCTTCGCACCCCAGCCCGACCGACCCAGCCCTT4030.13485386926871057No Hit
GACTACGTACACACTCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGG4030.13485386926871057No Hit
GACTACGTACACACTGGCGCACCGCCGGCCCGTCTCGCCCGCTCTGTCGG3990.1335153693255968No Hit
GACTACGTACACACTCCCCAGTCAAACTCCCCACCTGCCACTGTCCCCGG3990.1335153693255968No Hit
GACTACGTACACACTATCGTTTATGGTCGGAACTACGACGGTATCTGATC3920.13117299442514774No Hit
GACTACGTACACACTAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGG3900.13050374445359086No Hit
GACTACGTACACACTCGCTTGGTGAATTCTGCTTCACAATGATAGGAAGA3880.129834494482034No Hit
GACTACGTACACACTGCTACTACCACCAAGATCTGCACCCGCGGCGGCTC3860.12916524451047712No Hit
GACTACGTACACACTCCTGTGGTAACTTTTCTGACACCTCCTGCTTGAAA3820.12782674456736337No Hit
GACTACGTACACACTGTTCTTGATTAATGAAAACATTCTTGGCAAATGCT3750.12548436966691429No Hit
GACTACGTACACACTCCGGCCCGGACACGGAAAGGATTGACAGATTGATA3740.12514974468113585No Hit
GACTACGTACACACTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGC3740.12514974468113585No Hit
GACTACGTACACACTCCACCTGCCACTGTCCCCGGAGCGGGTCTCGCCCG3670.12280736978068679No Hit
GACTACGTACACACTCTTTTACTTCCTCTAGATAGTCAAGTTCGATCATC3640.12180349482335147No Hit
GACTACGTACACACTAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCAC3630.12146886983757303No Hit
GACTACGTACACACTCCGAAGTTTCCCTCAGGATAGCAGGCGCTCGGAGT3630.12146886983757303No Hit
GACTACGTACACACTGCCAGAACCGAGAGCCCGCGCGGGGCTCGCCTCCC3610.12079961986601614No Hit
GACTACGTACACACTGGCGCTCGGAGTCTCGCAGTTTTATCCGGTAAAGC3580.11979574490868085No Hit
GACTACGTACACACTGACAGATTGATAGCTCTTTCTCGATTCTGTGGGTG3560.11912649493712396No Hit
GACTACGTACACACTCCGGAACCCAAAGACTTTGGTTTCCCGGACGCTGC3520.11778799499401023No Hit
GACTACGTACACACTGCGACTTTTACTTCCTCTAGATAGTCAAGTTCGAT3520.11778799499401023No Hit
GACTACGTACACACTGTGTGTACAAAGGGCAGGGACTTAATCAACGCGAG3500.11711874502245334No Hit
GACTACGTACACACTCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACG3440.11511099510778272No Hit
GACTACGTACACACTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAA3440.11511099510778272No Hit
GACTACGTACACACTGTGAACAATCCAACGCTTGGTGAATTCTGCTTCAC3430.11477637012200427No Hit
GACTACGTACACACTCCCTCCTCCACTTCGGCCTTCAAAGTTCTCGTTTG3370.11276862020733365No Hit
GACTACGTACACACTCGCAGTTTTATCCGGTAAAGCGAATGACTAGAGGT3370.11276862020733365No Hit
GACTACGTACACACTGCCTTGTTACGACTTTTACTTCCTCTAGATAGTCA3340.11176474524999833No Hit
GACTACGTACACACTGCGGTATTCAGGCGACCGGGCCTGCTTTGAACACT3300.11042624530688458No Hit
GACTACGTACACACTGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT3260.10908774536377082No Hit
GACTACGTACACACTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTT3260.10908774536377082No Hit
GACTACGTACACACTCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAG3250.10875312037799238No Hit
GACTACGTACACACTGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTG3220.10774924542065707No Hit
GACTACGTACACACTTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAAT3170.10607612049176487No Hit
GACTACGTACACACTGTACAAAGGGCAGGGACTTAATCAACGCGAGCTTA3150.10540687052020802No Hit
GACTACGTACACACTCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCC3140.10507224553442956No Hit
GACTACGTACACACTGTAACTTTTCTGACACCTCCTGCTTGAAACCCAAA3100.1037337455913158No Hit
GACTACGTACACACTCCGGATTTTCAAGGGCCGACGAGAGCTCACCGGAC3080.10306449561975893No Hit
GACTACGTACACACTGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCG3080.10306449561975893No Hit
GACTACGTACACACTCCCGCTTCGCACCCCAGCCCGACCGACCCAGCCCT3060.10239524564820207No Hit
GACTACGTACACACTGCCAGAGGAAACTCTGGTGGAGGTCCGCAGCGGTC3050.10206062066242362No Hit
GACTACGTACACACTGGTAATTCTAGAGCTAATACATGCAAACGAGCGCT3030.10139137069086675No Hit
GACTACGTACACACTGTCCCTCTAAGAAGTTGGCCGCCGACCGCTCGGGG3030.10139137069086675No Hit
GACTACGTACACACTCCGTTGCCGGCGACGGCCGGGTATGGGCCCGACGC3010.10072212071930986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACA297400.0476.048655
CGTACAC297900.0474.68716
GACTACG299900.0474.075871
ACTACGT300700.0472.098082
CTACGTA302300.0469.045043
GTACACA304900.0464.024637
TACGTAC310450.0456.114654
ACACACT328800.0428.03839
TACACAC335100.0420.705388
TACACAT2650.0225.842218
ACACATG1500.0191.514199
CGACACA1150.0187.350856
TATACAC601.3202389E-6159.595178
TAGTACA1200.0159.595174
CTACACA1400.0136.795856
TCGTACA1600.0134.658424
GTACATA1101.3460522E-9130.577857
CGTACAT1151.8335413E-9124.9005666
ACATACT1151.8335413E-9124.9005669
GTATACA805.5249384E-6119.696377
Produced by FastQC (version 0.11.4)