FastQCFastQC Report
Thu 17 Oct 2019
male_brain.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemale_brain.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294099
Sequences flagged as poor quality0
Sequence length52-1200
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGAGTAGACTCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTG27330.9292789162832924No Hit
GACTACGAGTAGACTCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAG10460.35566254900560695No Hit
GACTACGAGTAGACTCCGCTTCGCACCCCAGCCCGACCGACCCAGCCCTT9380.31894022080999934No Hit
GACTACGAGTAGACTGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTG8980.3053393585153299No Hit
GACTACGAGTAGACTGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTA8230.2798377417128246No Hit
GACTACGAGTAGACTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGAT7640.2597764698281871No Hit
GACTACGAGTAGACTGTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACAC7200.24481552130405068No Hit
GACTACGAGTAGACTGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGG7200.24481552130405068No Hit
GACTACGAGTAGACTGTAACATATGCTTGTCTCAAAGATTAAGCCATGCA7040.2393751763861829No Hit
GACTACGAGTAGACTCTCACCCGGCCCGGACACGGAAAGGATTGACAGAT6610.2247542494194132No Hit
GACTACGAGTAGACTCCTCACCCGGCCCGGACACGGAAAGGATTGACAGA6350.21591368892787802No Hit
GACTACGAGTAGACTGTTGCCGGCGACGGCCGGGTATGGGCCCGACGCTC6110.20775317155107637No Hit
GACTACGAGTAGACTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATAT5770.19619243860060728No Hit
GACTACGAGTAGACTCGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCA5400.183611640978038No Hit
GACTACGAGTAGACTCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAA5370.1825915763059378No Hit
GACTACGAGTAGACTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAA5210.17715123138807No Hit
GACTACGAGTAGACTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTC5080.1727309511423024No Hit
GACTACGAGTAGACTGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTA4830.16423041220813397No Hit
GACTACGAGTAGACTGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGA4390.14926946368399757No Hit
GACTACGAGTAGACTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGA4330.14722933433979715No Hit
GACTACGAGTAGACTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG4330.14722933433979715No Hit
GACTACGAGTAGACTCCCGCTTCGCACCCCAGCCCGACCGACCCAGCCCT4310.14654929122506366No Hit
GACTACGAGTAGACTAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGG4180.1421290109792961No Hit
GACTACGAGTAGACTCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAA4100.1394088385203622No Hit
GACTACGAGTAGACTGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGC3980.13532857983196134No Hit
GACTACGAGTAGACTAGCAACTTTAATATACGCTATTGGAGCTGGAATTA3920.13328845048776092No Hit
GACTACGAGTAGACTCGCTTGGTGAATTCTGCTTCACAATGATAGGAAGA3910.13294842893039419No Hit
GACTACGAGTAGACTCCCGCTTTCACGGTCTGTACTCATACTGAAAATCA3910.13294842893039419No Hit
GACTACGAGTAGACTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCA3840.13056827802882703No Hit
GACTACGAGTAGACTTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATT3750.1275080840125264No Hit
GACTACGAGTAGACTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT3650.12410786843885901No Hit
GACTACGAGTAGACTCGACTTTTACTTCCTCTAGATAGTCAAGTTCGATC3510.1193475666357247No Hit
GACTACGAGTAGACTCCAAGATCTGCACCCGCGGCGGCTCCACCCGGGCC3450.11730743729152428No Hit
GACTACGAGTAGACTGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATAC3440.11696741573415755No Hit
GACTACGAGTAGACTCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCC3430.1166273941767908No Hit
GACTACGAGTAGACTGCAACTTTAATATACGCTATTGGAGCTGGAATTAC3410.11594735106205735No Hit
GACTACGAGTAGACTGGAATGAGTACACTTTAAATCCTTTAACGAGGATC3360.11424724327522365No Hit
GACTACGAGTAGACTCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCT3350.1139072217178569No Hit
GACTACGAGTAGACTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTG3340.11356720016049017No Hit
GACTACGAGTAGACTGGAAACTCTGGTGGAGGTCCGCAGCGGTCCTGACG3320.1128871570457567No Hit
GACTACGAGTAGACTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGC3300.11220711393102323No Hit
GACTACGAGTAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATGG3280.11152707081628975No Hit
GACTACGAGTAGACTAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCG3260.11084702770155627No Hit
GACTACGAGTAGACTCCCAGTCAAACTCCCCACCTGCCACTGTCCCCGGA3240.11016698458682281No Hit
GACTACGAGTAGACTCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGG3200.10880689835735585No Hit
GACTACGAGTAGACTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTG3140.10676676901315543No Hit
GACTACGAGTAGACTAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGG3090.10506666122632176No Hit
GACTACGAGTAGACTCTCATACTGAAAATCAAGATCAAGCGAGCTTTTGC3070.10438661811158828No Hit
GACTACGAGTAGACTCGCAGTTTTATCCGGTAAAGCGAATGACTAGAGGT3040.10336655343948806No Hit
GACTACGAGTAGACTGTTTGACTGGGGCGGTACACCTGTCAAACGGTAAC3040.10336655343948806No Hit
GACTACGAGTAGACTGTTATTTTTCGTCACTACCTCCCCGCGTCAGGAGT3040.10336655343948806No Hit
GACTACGAGTAGACTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTT3000.10200646721002112No Hit
GACTACGAGTAGACTGCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT3000.10200646721002112No Hit
GACTACGAGTAGACTGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGC2980.10132642409528764No Hit
GACTACGAGTAGACTCTCGTCGCGGCCTAGCCCTCGCGGCTCCCGTTGCC2950.10030635942318743No Hit
GACTACGAGTAGACTGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCG2950.10030635942318743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT292650.0452.517644
GACTACG296200.0448.56331
GAGTAGA295000.0448.214057
GTAGACT292350.0447.26299
AGTAGAC293050.0446.897988
CTACGAG301700.0439.247283
CGAGTAG303050.0436.383646
ACGAGTA303450.0436.33675
ACTACGA315250.0420.73092
AGTAGAT4350.0310.64818
GTAGATG4250.0188.618829
TAGAGTA5050.0185.950964
CTCGAGT3400.0175.146043
TCGAGTA3600.0165.415714
ACTCGAG5100.0116.764042
GTAGATT1255.7242505E-791.614879
GTAGATA2800.089.9788749
GGTAGAC2350.087.716367
GTAGATC2253.45608E-1181.435439
CGGTAGA2551.8189894E-1280.836646
Produced by FastQC (version 0.11.4)