FastQCFastQC Report
Thu 17 Oct 2019
male_testis.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemale_testis.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377689
Sequences flagged as poor quality0
Sequence length56-1201
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGCGTCTAGTCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTG40831.0810481639655907No Hit
GACTACGCGTCTAGTCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAG39211.0381557313027385No Hit
GACTACGCGTCTAGTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGAT22450.5944043909142178No Hit
GACTACGCGTCTAGTCTGCCAGTAACATATGCTTGTCTCAAAGATTAAGC20630.5462165961942234No Hit
GACTACGCGTCTAGTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTC19670.5207988583199404No Hit
GACTACGCGTCTAGTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCA19620.519475017805655No Hit
GACTACGCGTCTAGTCTCACCCGGCCCGGACACGGAAAGGATTGACAGAT19210.5086195255885133No Hit
GACTACGCGTCTAGTGTAACATATGCTTGTCTCAAAGATTAAGCCATGCA18640.4935277437256579No Hit
GACTACGCGTCTAGTCCTCACCCGGCCCGGACACGGAAAGGATTGACAGA15260.40403612495995384No Hit
GACTACGCGTCTAGTAGCAACTTTAATATACGCTATTGGAGCTGGAATTA13230.35028820007996No Hit
GACTACGCGTCTAGTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTG12530.3317544328799621No Hit
GACTACGCGTCTAGTGTGAAACTGCGAATGGCTCATTAAATCAGTTATGG12340.32672383892567697No Hit
GACTACGCGTCTAGTGTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACAC12030.31851602773710647No Hit
GACTACGCGTCTAGTGCAACTTTAATATACGCTATTGGAGCTGGAATTAC11680.3092491441371075No Hit
GACTACGCGTCTAGTGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGC11480.30395378207996526No Hit
GACTACGCGTCTAGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAA11390.30157086915425124No Hit
GACTACGCGTCTAGTGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTA11040.2923039855542523No Hit
GACTACGCGTCTAGTCGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCA10590.28038942092568225No Hit
GACTACGCGTCTAGTCCTGCCAGTAACATATGCTTGTCTCAAAGATTAAG10520.27853604420568245No Hit
GACTACGCGTCTAGTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCT10290.2724463778399689No Hit
GACTACGCGTCTAGTCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAA9910.2623851899313986No Hit
GACTACGCGTCTAGTCGACTTTTACTTCCTCTAGATAGTCAAGTTCGATC9630.25497168305139944No Hit
GACTACGCGTCTAGTGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATAC9520.2520592339199712No Hit
GACTACGCGTCTAGTGTCTGACGCGCGCGCGAGTCCGAGGGTTCGAGCGA8780.23246639430854485No Hit
GACTACGCGTCTAGTCTGACGCGCGCGCGAGTCCGAGGGTTCGAGCGAAA8690.23008348138283086No Hit
GACTACGCGTCTAGTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATAT8680.22981871327997375No Hit
GACTACGCGTCTAGTCTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGT8480.2245233512228315No Hit
GACTACGCGTCTAGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGC8390.2221404382971175No Hit
GACTACGCGTCTAGTGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTA7630.20201806247997692No Hit
GACTACGCGTCTAGTGGAATGAGTACACTTTAAATCCTTTAACGAGGATC7450.1972522366285489No Hit
GACTACGCGTCTAGTGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGG7180.19010349785140684No Hit
GACTACGCGTCTAGTCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAAC7070.1871910487199786No Hit
GACTACGCGTCTAGTGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGA7060.1869262806171215No Hit
GACTACGCGTCTAGTCCGGAACCCAAAGACTTTGGTTTCCCGGACGCTGC7020.18586720820569305No Hit
GACTACGCGTCTAGTATTGGAATGAGTACACTTTAAATCCTTTAACGAGG6770.17924800563426524No Hit
GACTACGCGTCTAGTGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTA6690.17712986081140833No Hit
GACTACGCGTCTAGTCTCGTTTGAATATTTGCTACTACCACCAAGATCTG6300.16680390479998092No Hit
GACTACGCGTCTAGTGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTG6280.16627436859426672No Hit
GACTACGCGTCTAGTGTTGCCGGCGACGGCCGGGTATGGGCCCGACGCTC6280.16627436859426672No Hit
GACTACGCGTCTAGTGCTTTGCAACCATACTCCCCCCGGAACCCAAAGAC6130.16230284705141002No Hit
GACTACGCGTCTAGTGCGGTATTCAGGCGACCGGGCCTGCTTTGAACACT6110.1617733108456958No Hit
GACTACGCGTCTAGTGTGTGTACAAAGGGCAGGGACTTAATCAACGCGAG6060.16044947033141024No Hit
GACTACGCGTCTAGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGC6050.16018470222855313No Hit
GACTACGCGTCTAGTCCCGGCCCGGACACGGAAAGGATTGACAGATTGAT5970.15806655740569622No Hit
GACTACGCGTCTAGTGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACG5880.1556836444799822No Hit
GACTACGCGTCTAGTGGAGTCTGACGCGCGCGCGAGTCCGAGGGTTCGAG5880.1556836444799822No Hit
GACTACGCGTCTAGTGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT5850.15488934017141087No Hit
GACTACGCGTCTAGTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTC5740.15197689103998263No Hit
GACTACGCGTCTAGTGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTA5630.1490644419085544No Hit
GACTACGCGTCTAGTGCGCAAATTACCCACTCCTGACGCGGGGAGGTAGT5540.1466815289828404No Hit
GACTACGCGTCTAGTGTTGAGATCGTTTCGGCCCCAAGACCTCTAGTCAT5510.14588722467426904No Hit
GACTACGCGTCTAGTCGAATGTCTGCCCTATCAACTTTCGATGGTACTTT5340.1413861669256981No Hit
GACTACGCGTCTAGTGCGACTTTTACTTCCTCTAGATAGTCAAGTTCGAT5330.141121398822841No Hit
GACTACGCGTCTAGTCGAAAAATAACAATACAGGACTCTTTCGAGGCCCT5260.13926802210284123No Hit
GACTACGCGTCTAGTCAGATTGATAGCTCTTTCTCGATTCTGTGGGTGGT5260.13926802210284123No Hit
GACTACGCGTCTAGTATCGTTTATGGTCGGAACTACGACGGTATCTGATC5240.138738485897127No Hit
GACTACGCGTCTAGTGTACAAAGGGCAGGGACTTAATCAACGCGAGCTTA5230.13847371779426987No Hit
GACTACGCGTCTAGTCTTAATCATGGCCCCAGTTCGGGAAACCCACAAAA5230.13847371779426987No Hit
GACTACGCGTCTAGTCCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGA5190.13741464538284145No Hit
GACTACGCGTCTAGTCGCTTGGTGAATTCTGCTTCACAATGATAGGAAGA5170.1368851091771272No Hit
GACTACGCGTCTAGTGGAAACTCTGGTGGAGGTCCGCAGCGGTCCTGACG5150.13635557297141299No Hit
GACTACGCGTCTAGTGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTG5120.13556126866284163No Hit
GACTACGCGTCTAGTGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCG5070.1342374281485561No Hit
GACTACGCGTCTAGTGTTTATGGTCGGAACTACGACGGTATCTGATCGTC5050.13370789194284186No Hit
GACTACGCGTCTAGTGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG4990.1321192833256992No Hit
GACTACGCGTCTAGTAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGG4940.13079544281141361No Hit
GACTACGCGTCTAGTCCGGCCCGGACACGGAAAGGATTGACAGATTGATA4850.12841252988569962No Hit
GACTACGCGTCTAGTACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGG4790.12682392126855693No Hit
GACTACGCGTCTAGTCCCGCTTTCACGGTCTGTACTCATACTGAAAATCA4770.1262943850628427No Hit
GACTACGCGTCTAGTTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATT4660.12338193593141447No Hit
GACTACGCGTCTAGTGCTCTTTCTCGATTCTGTGGGTGGTGGTGCATGGC4650.12311716782855736No Hit
GACTACGCGTCTAGTAAGAAGTTGGCCGCCGACCGCTCGGGGCCCCGTAA4630.12258763162284314No Hit
GACTACGCGTCTAGTGTAACTTTTCTGACACCTCCTGCTTGAAACCCAAA4630.12258763162284314No Hit
GACTACGCGTCTAGTATTGATAGCTCTTTCTCGATTCTGTGGGTGGTGGT4620.12232286351998602No Hit
GACTACGCGTCTAGTGTCGGCATCGTTTATGGTCGGAACTACGACGGTAT4520.1196751824914149No Hit
GACTACGCGTCTAGTCAGTAACATATGCTTGTCTCAAAGATTAAGCCATG4500.11914564628570067No Hit
GACTACGCGTCTAGTCGTTTATGGTCGGAACTACGACGGTATCTGATCGT4440.117557037668558No Hit
GACTACGCGTCTAGTGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA4400.11649796525712953No Hit
GACTACGCGTCTAGTGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCG4370.11570366094855819No Hit
GACTACGCGTCTAGTCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACG4370.11570366094855819No Hit
GACTACGCGTCTAGTCTCGTTCATGGGGAATAATTGCAATCCCCGATCCC4340.11490935663998687No Hit
GACTACGCGTCTAGTGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA4330.11464458853712976No Hit
GACTACGCGTCTAGTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGA4300.11385028422855842No Hit
GACTACGCGTCTAGTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACT4290.11358551612570131No Hit
GACTACGCGTCTAGTCTTTTACTTCCTCTAGATAGTCAAGTTCGATCATC4270.11305597991998707No Hit
GACTACGCGTCTAGTGACAGATTGATAGCTCTTTCTCGATTCTGTGGGTG4260.11279121181712996No Hit
GACTACGCGTCTAGTCCCAGTCAAACTCCCCACCTGCCACTGTCCCCGGA4230.11199690750855863No Hit
GACTACGCGTCTAGTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTG4210.11146737130284441No Hit
GACTACGCGTCTAGTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCC4110.10881969027427328No Hit
GACTACGCGTCTAGTGGTAATTCTAGAGCTAATACATGCAAACGAGCGCT4110.10881969027427328No Hit
GACTACGCGTCTAGTGTGAATTCTGCTTCACAATGATAGGAAGAGCCGAC4070.10776061786284483No Hit
GACTACGCGTCTAGTGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC4040.10696631355427348No Hit
GACTACGCGTCTAGTATTAAATCAGTTATGGTCCCTTTGATCGCTCCCAT4020.10643677734855927No Hit
GACTACGCGTCTAGTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAG4020.10643677734855927No Hit
GACTACGCGTCTAGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGC4010.10617200924570216No Hit
GACTACGCGTCTAGTGTTCGGGCGGGGGGGCCTCAGGGCGACGGCTTCTC3990.10564247303998793No Hit
GACTACGCGTCTAGTGTTCTTGATTAATGAAAACATTCTTGGCAAATGCT3950.10458340062855948No Hit
GACTACGCGTCTAGTGCTCAATCTCGTGTGGCTGAATTCCACTTGTCCCT3910.10352432821713103No Hit
GACTACGCGTCTAGTCGCAGTTTTATCCGGTAAAGCGAATGACTAGAGGT3870.10246525580570258No Hit
GACTACGCGTCTAGTGATAAAACTGCGAGACTCCGAGCGCCTGCTATCCT3860.10220048770284546No Hit
GACTACGCGTCTAGTCTCATACTGAAAATCAAGATCAAGCGAGCTTTTGC3850.10193571959998834No Hit
GACTACGCGTCTAGTCGACTTTCGTTCTTGATTAATGAAAACATTCTTGG3840.10167095149713123No Hit
GACTACGCGTCTAGTCAAAGGGCAGGGACTTAATCAACGCGAGCTTATGA3830.10140618339427411No Hit
GACTACGCGTCTAGTCACCCGGCCCGGACACGGAAAGGATTGACAGATTG3780.10008234287998856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG375500.0470.948463
ACTACGC376700.0470.703952
TACGCGT376550.0469.63524
ACGCGTC377100.0468.887545
CGCGTCT377600.0468.266666
GTCTAGT375200.0467.984079
GACTACG379900.0467.11261
GCGTCTA386700.0457.247227
CGTCTAG385000.0456.19468
CGTCTAT3150.0382.926648
TATGCGT352.3464963E-10337.876434
CTAGCGT1500.0331.118933
GACTAGC1700.0292.163761
GTCTATC1650.0286.683079
GTCTATG1450.0228.357869
TACGCTC1700.0222.600954
ACTAGCG2250.0220.745942
CGCTCTA1850.0204.552226
AGCGTCT2500.0198.671365
ACGCTCT2050.0184.59595
Produced by FastQC (version 0.11.4)