FastQCFastQC Report
Thu 17 Oct 2019
female_brain.trim.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefemale_brain.trim.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241886
Sequences flagged as poor quality0
Sequence length48-759
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACTCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGC57002.356481979114128No Hit
CACACTCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCAT25701.062483980056721No Hit
CACACTCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTC18330.7577949943361748No Hit
CACACTCTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGT16890.6982628180217128No Hit
CACACTCCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCT15260.6308757017768701No Hit
CACACTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTC14820.6126853145696733No Hit
CACACTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTT13330.5510860487998479No Hit
CACACTCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTA13200.5457116162159034No Hit
CACACTGTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGG11990.4956880513961122No Hit
CACACTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGTCT11820.4886599472478771No Hit
CACACTGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGA11740.4853526041192958No Hit
CACACTCCTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAG10650.4402900539923766No Hit
CACACTAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTG10510.43450220351735946No Hit
CACACTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTA9210.3807578776779144No Hit
CACACTGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGTCTGAGT8420.3480978642831747No Hit
CACACTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCCGCGG7470.3088231646312726No Hit
CACACTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCT6980.2885656879687125No Hit
CACACTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAA6740.27864365858296886No Hit
CACACTGCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTG5910.24432997362393855No Hit
CACACTCGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGA5780.23895554103999403No Hit
CACACTCGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTCG5760.23812870525784874No Hit
CACACTGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGC5670.23440794423819486No Hit
CACACTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTC5650.23358110845604954No Hit
CACACTCTCGCCTCGGCCGGCGCCTAGCAGCTGACTTAGAACTGGTGCGG5620.2323408547828316No Hit
CACACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTCCCTTTGA5530.22862009376317768No Hit
CACACTCCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTC5440.2248993327435238No Hit
CACACTCCAAGATCTGCACCCGCGGCGGCTCCACCCGGGCCCACGCCCTG5330.22035173594172464No Hit
CACACTGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGGGTCTCGCCC5120.2116699602291989No Hit
CACACTCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGA5090.21042970655598092No Hit
CACACTCCCGCTTTCACGGTCTGTACTCATACTGAAAATCAAGATCAAGC5040.20836261710061765No Hit
CACACTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAG4990.20629552764525436No Hit
CACACTGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCC4650.19223931934878413No Hit
CACACTGTTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT4630.1914124835666388No Hit
CACACTTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC4590.18975881200234823No Hit
CACACTAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGA4550.1881051404380576No Hit
CACACTGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCACAA4540.18769172254698493No Hit
CACACTGGTAAAGCGAATGACTAGAGGTCTTGGGGCCGAAACGATCTCAA4520.18686488676483964No Hit
CACACTCCCAGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGGGTCTC4470.18479779730947637No Hit
CACACTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTTTCGTCCGT4410.18231728996304042No Hit
CACACTATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGG4310.1781831110523139No Hit
CACACTGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCGAGCTTATG4300.17776969316124125No Hit
CACACTGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTG4290.1773562752701686No Hit
CACACTCTTGTTACGACTTTTTACTTCCTCTAGATAGTCAAGTTCGATCA4280.17694285737909593No Hit
CACACTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGT4240.17528918581480532No Hit
CACACTCGAATGTCTGCCCTATCAACTTTCGATGGTACTTTCTGTGCCTA4160.1719818426862241No Hit
CACACTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGGAGG4080.16867449955764285No Hit
CACACTCTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATT4040.16702082799335224No Hit
CACACTCAGATTGATAGCTCTTTCTCGATTCTGTGGGTGGTGGTGCATGG4030.16660741010227959No Hit
CACACTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTT4010.16578057432013427No Hit
CACACTGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGACTG3940.16288664908262568No Hit
CACACTCTCATACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCT3940.16288664908262568No Hit
CACACTGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGG3910.16164639540940776No Hit
CACACTGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTC3900.1612329775183351No Hit
CACACTAAGAAGTTGGCCGCCGACCGCTCGGGGCCCCGTAACTATTTAGC3840.15875247017189917No Hit
CACACTGGAAACTCTGGTGGAGGTCCGCAGCGGTCCTGACGTGCAAATCG3830.1583390522808265No Hit
CACACTGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAG3790.15668538071653587No Hit
CACACTGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTG3780.15627196282546324No Hit
CACACTCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCG3680.1521377839147367No Hit
CACACTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCACAATA3610.14924385867722811No Hit
CACACTGCAAGAGCGCGCGCGACGGTACCCGAAAGATGGTGAACTATGCC3550.14676335133079219No Hit
CACACTGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA3520.1455230976575742No Hit
CACACTAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGAC3510.14510967976650158No Hit
CACACTGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTAAGAGATGGG3470.14345600820221097No Hit
CACACTCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCAATCACGGT3440.14221575452899302No Hit
CACACTCCCCAGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGGGTCT3370.13932182929148443No Hit
CACACTCCGCTTCGCACCCCAGCCCGACCGACCCAGCCCTTAGAGCCAAT3330.1376681577271938No Hit
CACACTCCGAAGTTTCCCTCAGGATAGCAGGCGCTCGGAGTCTCGCAGTT3320.13725473983612113No Hit
CACACTCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCC3310.1368413219450485No Hit
CACACTCTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTCGGCG3310.1368413219450485No Hit
CACACTCGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC3290.13601448616290318No Hit
CACACTGGCGCTCGGAGTCTCGCAGTTTTATCCGGTAAAGCGAATGACTA3280.13560106827183052No Hit
CACACTGCGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTC3270.13518765038075786No Hit
CACACTGCTACTACCACCAAGATCTGCACCCGCGGCGGCTCCACCCGGGC3270.13518765038075786No Hit
CACACTCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT3220.13312056092539462No Hit
CACACTATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA3210.13270714303432196No Hit
CACACTCGCAGTTTTATCCGGTAAAGCGAATGACTAGAGGTCTTGGGGCC3200.1322937251432493No Hit
CACACTGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA3160.1306400535789587No Hit
CACACTGACAGATTGATAGCTCTTTCTCGATTCTGTGGGTGGTGGTGCAT3150.13022663568788603No Hit
CACACTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTTTCGTC3140.12981321779681337No Hit
CACACTGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCGAGCTTAT3110.1285729641235954No Hit
CACACTCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGACC3090.1277461283414501No Hit
CACACTGGCGCACCGCCGGCCCGTCTCGCCCGCTCTGTCGGGGAGGTGGA3080.12733271045037745No Hit
CACACTGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGG3070.12691929255930479No Hit
CACACTGCCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATC3050.12609245677715947No Hit
CACACTCCCTCCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGC3050.12609245677715947No Hit
CACACTCCACCTGCCACTGTCCCCGGAGCGGGTCTCGCCCGGCGCGCGCC3030.1252656209950142No Hit
CACACTCCGGAACCCAAAGACTTTGGTTTCCCGGACGCTGCCCGGCGGGT3000.12402536732179623No Hit
CACACTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACC2910.12030460630214233No Hit
CACACTGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTTTC2910.12030460630214233No Hit
CACACTAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACTGTGAAGAG2890.11947777051999701No Hit
CACACTGCGGTATTCAGGCGACCGGGCCTGCTTTGAACACTCTAATTTTT2860.11823751684677905No Hit
CACACTGCCAGAACCGAGAGCCCGCGCGGGGCTCGCCTCCCCGCCTCACC2800.11575700950034315No Hit
CACACTCCGTTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCC2760.11410333793605253No Hit
CACACTCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAA2750.11368992004497987No Hit
CACACTTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTAC2750.11368992004497987No Hit
CACACTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGT2730.11286308426283456No Hit
CACACTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACT2710.11203624848068926No Hit
CACACTGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC2700.1116228305896166No Hit
CACACTCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCT2700.1116228305896166No Hit
CACACTCCCGCTTCGCACCCCAGCCCGACCGACCCAGCCCTTAGAGCCAA2660.10996915902532597No Hit
CACACTGCCTCGGCCGGCGCCTAGCAGCTGACTTAGAACTGGTGCGGACC2630.10872890535210801No Hit
CACACTCCCATCTCTTAGGACCGACTGACCCATGTTCAACTGCTGTTCAC2620.10831548746103535No Hit
CACACTATTGATAGCTCTTTCTCGATTCTGTGGGTGGTGGTGCATGGCCG2590.10707523378781739No Hit
CACACTCAGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGTCTGA2560.10583498011459945No Hit
CACACTGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA2550.10542156222352679No Hit
CACACTGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCA2550.10542156222352679No Hit
CACACTCTCCTACTCGTCGCGGCCTAGCCCTCGCGGCTCCCGTTGCCGGC2510.10376789065923617No Hit
CACACTGCCAGAGGAAACTCTGGTGGAGGTCCGCAGCGGTCCTGACGTGC2510.10376789065923617No Hit
CACACTGGTAATTCTAGAGCTAATACATGCAAACGAGCGCTGACCTCCGG2510.10376789065923617No Hit
CACACTCCGGATTTTCAAGGGCCGACGAGAGCTCACCGGACGCCGCCGGA2500.10335447276816352No Hit
CACACTGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTG2500.10335447276816352No Hit
CACACTCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGAC2490.10294105487709086No Hit
CACACTAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGT2470.10211421909494556No Hit
CACACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAG2470.10211421909494556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACTA30800.0470.26461
ACACTGT35400.0469.646762
ACACTGG27600.0467.253142
ACACTCG22100.0462.693272
ACACTGC26200.0458.242922
CACTCTC13350.0457.771763
CACACTT11400.0451.653561
CACTGTT12400.0450.155763
ACACTAG7050.0447.073272
CACTCTT11650.0446.09193
ACTGTCG3700.0442.18474
ACTCTCA4550.0438.897584
ACACTAT9000.0438.427582
CACTCCC17950.0428.92573
ACTCTCG4100.0428.386234
ACTGTTC3900.0425.67784
CACTCGG6650.0423.312163
CACACTG111900.0422.932251
CACTATT4850.0421.670963
ACACTAA10550.0417.344942
Produced by FastQC (version 0.11.4)