FastQCFastQC Report
Thu 17 Oct 2019
female_ovary.trim.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamefemale_ovary.trim.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272740
Sequences flagged as poor quality0
Sequence length44-762
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGTG167350.0422.113981
CTGTGTC86350.0407.124851
CTGTGTA35550.0354.40951
TGTGTGG63900.0345.603942
TGTGTCT38600.0328.850622
TGTGTGC53850.0322.912932
TGTGTGT77500.0304.135742
GTGTGTT27600.0301.765323
TGTGTCG24000.0288.044832
CTGTGTT31350.0279.968441
GTGTGGG25350.0272.705173
TGTGTCC33450.0268.74012
TGTGTAT16800.0258.411742
GTGTGTC22850.0251.94593
TGTGTGA32100.0236.676162
GTGTGGC25050.0233.586443
GTGTGCT19050.0226.652113
GTGTGCG16450.0226.618233
TGTGTCA29200.0224.62892
GTGTGCC24700.0223.523183
Produced by FastQC (version 0.11.4)