FastQCFastQC Report
Thu 17 Oct 2019
male_brain.trim.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemale_brain.trim.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198859
Sequences flagged as poor quality0
Sequence length43-755
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGACTCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGC22621.137489376895187No Hit
TAGACTCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCAT8690.43699304532357097No Hit
TAGACTGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGACTG8180.4113467331124063No Hit
TAGACTGTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGG6440.323847550274315No Hit
TAGACTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTC6150.3092643531346331No Hit
TAGACTGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGTCTGAGT5740.28864672959232424No Hit
TAGACTCCGCTTCGCACCCCAGCCCGACCGACCCAGCCCTTAGAGCCAAT5710.28713812299166747No Hit
TAGACTCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTC5390.2710463192513288No Hit
TAGACTGTTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT5260.26450902398181625No Hit
TAGACTCCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCT5160.25948033531296044No Hit
TAGACTGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGGGTCTCGCCC5030.25294304004344786No Hit
TAGACTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTA4850.2438914004395074No Hit
TAGACTCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTA4690.2358454985693381No Hit
TAGACTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTT4220.2122106618257157No Hit
TAGACTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAA4170.20969631749128778No Hit
TAGACTGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGA3960.19913607128669059No Hit
TAGACTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGT3950.19863320241980498No Hit
TAGACTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCCGCGG3760.18907869394897894No Hit
TAGACTAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGA3720.1870672184814366No Hit
TAGACTCGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGA3700.18606148074766543No Hit
TAGACTGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGACTGG3680.18505574301389427No Hit
TAGACTGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTAAGAGATGGG3670.1845528741470087No Hit
TAGACTTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC3290.16544385720535656No Hit
TAGACTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGTCT3190.16041516853650073No Hit
TAGACTCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGA3090.1553864798676449No Hit
TAGACTGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA3030.15236926666633144No Hit
TAGACTCCCGCTTTCACGGTCTGTACTCATACTGAAAATCAAGATCAAGC2980.1498549223319035No Hit
TAGACTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTC2940.14784344686436118No Hit
TAGACTCGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC2870.14432336479616212No Hit
TAGACTAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGT2860.14382049592927654No Hit
TAGACTCCCGCTTCGCACCCCAGCCCGACCGACCCAGCCCTTAGAGCCAA2830.14231188932861977No Hit
TAGACTCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCG2820.1418090204617342No Hit
TAGACTAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTG2800.14080328272796303No Hit
TAGACTAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGAC2780.13979754499419186No Hit
TAGACTCGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTCG2750.13828893839353512No Hit
TAGACTCCAAGATCTGCACCCGCGGCGGCTCCACCCGGGCCCACGCCCTG2650.1332602497246793No Hit
TAGACTGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGC2630.13225451199090812No Hit
TAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTCCCTTTGA2600.13074590539025138No Hit
TAGACTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTTTCGTCCGT2590.1302430365233658No Hit
TAGACTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGGAGG2570.12923729878959464No Hit
TAGACTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACC2520.12672295445516674No Hit
TAGACTCGCAGTTTTATCCGGTAAAGCGAATGACTAGAGGTCTTGGGGCC2490.12521434785451No Hit
TAGACTGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTC2490.12521434785451No Hit
TAGACTCTCGTCGCGGCCTAGCCCTCGCGGCTCCCGTTGCCGGCGACGGC2480.1247114789876244No Hit
TAGACTGCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTG2470.1242086101207388No Hit
TAGACTCTCATACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCT2440.12270000352008208No Hit
TAGACTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCACAATA2420.1216942657863109No Hit
TAGACTGTTATTTTTCGTCACTACCTCCCCGCGTCAGGAGTGGGTAATTT2390.12018565918565415No Hit
TAGACTCCCAGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGGGTCTC2280.11465410164991274No Hit
TAGACTGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTG2280.11465410164991274No Hit
TAGACTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCT2190.11012828184794252No Hit
TAGACTGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGA2180.10962541298105692No Hit
TAGACTGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGG2020.10157951111088762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTC68150.0456.54371
TAGACTG91150.0454.95441
TAGACTA29200.0431.152861
AGACTGT33150.0426.110262
AGACTGG31400.0423.919342
AGACTCG17350.0423.842442
AGACTCT23350.0415.590882
TAGACTT11250.0413.708371
AGACTGC23800.0403.821962
AGACTAT8950.0397.818732
AGACTCA10450.0385.253572
GACTGCC10500.0383.4193
GACTAGC3350.0382.062743
AGACTGA12050.0366.930152
GACTGTC10200.0355.91093
ACTCTCG3850.0344.533144
GACTGGC11800.0343.150053
GACTGTG10600.0340.28493
GACTCGC8150.0339.786563
GACTCGA4600.0338.948553
Produced by FastQC (version 0.11.4)