FastQCFastQC Report
Thu 17 Oct 2019
male_testis.trim.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamemale_testis.trim.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278631
Sequences flagged as poor quality0
Sequence length47-813
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAGTCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGC35961.2905958059225282No Hit
TCTAGTCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCAT34691.245015809439725No Hit
TCTAGTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTC19990.7174363225915278No Hit
TCTAGTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTT17770.6377610531491471No Hit
TCTAGTCTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGT16840.6043835754097713No Hit
TCTAGTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGTCT16580.5950522375471502No Hit
TCTAGTCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTC16370.5875153877350331No Hit
TCTAGTGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGTCTGAGT15000.5383464151512215No Hit
TCTAGTCCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCT12670.4547232719977317No Hit
TCTAGTAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTG11480.41201445639573486No Hit
TCTAGTGTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGG11260.404118708973517No Hit
TCTAGTGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGC10150.36428107425232653No Hit
TCTAGTGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTCCCTTTGA10110.3628454838119233No Hit
TCTAGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAA9880.35459083877960457No Hit
TCTAGTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCT9570.34346501286647935No Hit
TCTAGTGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGA9370.336287060664463No Hit
TCTAGTCCTGCCAGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAG8820.31654769210891825No Hit
TCTAGTCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTA8740.31367651122811174No Hit
TCTAGTCGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTCG8200.29429604028266776No Hit
TCTAGTCGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGA8110.2910659617917604No Hit
TCTAGTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTTTCGTCCGT7910.28388800958974414No Hit
TCTAGTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTA7780.27922234065843354No Hit
TCTAGTCTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATT7460.2677376171352075No Hit
TCTAGTGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCGAGCTTATG7270.26091856254329204No Hit
TCTAGTGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGG7070.25374061034127576No Hit
TCTAGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCACAATA5880.21103179473927883No Hit
TCTAGTGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGACTG5860.21031399951907723No Hit
TCTAGTCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCC5700.20457163775746418No Hit
TCTAGTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGGAGG5590.2006237640463552No Hit
TCTAGTGTTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT5500.19739368555544787No Hit
TCTAGTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCCGCGG5450.1955991975049438No Hit
TCTAGTCCGGAACCCAAAGACTTTGGTTTCCCGGACGCTGCCCGGCGGGT5400.19380470945443976No Hit
TCTAGTATTGGAATGAGTACACTTTAAATCCTTTAACGAGGATCCATTGG5380.19308691423423813No Hit
TCTAGTGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA5240.1880623476928267No Hit
TCTAGTGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTC5030.18052549788070962No Hit
TCTAGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACT4920.17657762416960066No Hit
TCTAGTGCGGTATTCAGGCGACCGGGCCTGCTTTGAACACTCTAATTTTT4890.17550093133929823No Hit
TCTAGTGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA4800.1722708528483909No Hit
TCTAGTGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCA4760.17083526240798763No Hit
TCTAGTGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGGGTCTCGCCC4730.16975856957768518No Hit
TCTAGTGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCACAA4690.16832297913728195No Hit
TCTAGTCGAATGTCTGCCCTATCAACTTTCGATGGTACTTTCTGTGCCTA4650.16688738869687866No Hit
TCTAGTCGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGA4650.16688738869687866No Hit
TCTAGTCCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTC4640.16652849108677786No Hit
TCTAGTGCGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTC4630.16616959347667704No Hit
TCTAGTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTTTCGTC4600.1650929006463746No Hit
TCTAGTAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGA4580.16437510542617295No Hit
TCTAGTCCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTG4460.1600683341049632No Hit
TCTAGTGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC4430.15899164127466076No Hit
TCTAGTCAGATTGATAGCTCTTTCTCGATTCTGTGGGTGGTGGTGCATGG4390.1575560508342575No Hit
TCTAGTGCGCAAATTACCCACTCCTGACGCGGGGAGGTAGTGACGAAAAA4350.15612046039385424No Hit
TCTAGTACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGT4280.15360817712314853No Hit
TCTAGTCCCGCTTTCACGGTCTGTACTCATACTGAAAATCAAGATCAAGC4120.1478658153615355No Hit
TCTAGTCGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC4110.14750691775143468No Hit
TCTAGTGTGAACAGCAGTTGAACATGGGTCAGTCGGTCCTAAGAGATGGG4080.14643022492113225No Hit
TCTAGTATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA4060.14571242970093062No Hit
TCTAGTCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT4060.14571242970093062No Hit
TCTAGTGTGTGTACAAAGGGCAGGGACTTAATCAACGCGAGCTTATGACC4040.144994634480729No Hit
TCTAGTCGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGT4030.14463573687062817No Hit
TCTAGTTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTAC4020.14427683926052737No Hit
TCTAGTGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCC3940.14140565837972083No Hit
TCTAGTGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGT3930.14104676076962003No Hit
TCTAGTGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCT3920.14068786315951923No Hit
TCTAGTGGAAACTCTGGTGGAGGTCCGCAGCGGTCCTGACGTGCAAATCG3870.13889337510901514No Hit
TCTAGTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTC3860.13853447749891434No Hit
TCTAGTCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGACC3850.13817557988881352No Hit
TCTAGTGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCT3810.13673998944841026No Hit
TCTAGTGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTC3770.135304399008007No Hit
TCTAGTCAGTAACATATGCTTGTCTCAAAGATTAAGCCATGCAAGTCTGA3740.13422770617770458No Hit
TCTAGTATTGATAGCTCTTTCTCGATTCTGTGGGTGGTGGTGCATGGCCG3700.1327921157373013No Hit
TCTAGTGCTCTTTCTCGATTCTGTGGGTGGTGGTGCATGGCCGTTCTTAG3660.13135652529689804No Hit
TCTAGTGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAG3650.13099762768679724No Hit
TCTAGTGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCGAGCTTAT3640.13063873007669644No Hit
TCTAGTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAG3620.12992093485649478No Hit
TCTAGTGACAGATTGATAGCTCTTTCTCGATTCTGTGGGTGGTGGTGCAT3620.12992093485649478No Hit
TCTAGTCTTAATCATGGCCCCAGTTCGGGAAACCCACAAAATAGAACCGG3610.12956203724639398No Hit
TCTAGTCGCAGTTTTATCCGGTAAAGCGAATGACTAGAGGTCTTGGGGCC3500.125614163535285No Hit
TCTAGTCTCATACTGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCT3490.1252552659251842No Hit
TCTAGTGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCACAATACG3470.12453747070498258No Hit
TCTAGTGTTCGGGCGGGGGGGCCTCAGGGCGACGGCTTCTCCAGCCGCGG3440.12346077787468014No Hit
TCTAGTGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGA3430.12310188026457931No Hit
TCTAGTCTTTTACTTCCTCTAGATAGTCAAGTTCGATCATCTTCTCGGCG3370.12094849460397444No Hit
TCTAGTGTACAAAGGGCAGGGACTTAATCAACGCGAGCTTATGACCCGCA3360.12058959699387362No Hit
TCTAGTCCACTCCTGACGCGGGGAGGTAGTGACGAAAAATAACAATACAG3340.119871801773672No Hit
TCTAGTGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTC3290.11807731372316792No Hit
TCTAGTGCCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATC3280.1177184161130671No Hit
TCTAGTCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACC3270.1173595185029663No Hit
TCTAGTCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCAATCACGGT3210.1152061328423614No Hit
TCTAGTGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA3210.1152061328423614No Hit
TCTAGTGTCTGCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTG3160.11341164479185732No Hit
TCTAGTGACGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTT3140.11269384957165571No Hit
TCTAGTGATTGATAGCTCTTTCTCGATTCTGTGGGTGGTGGTGCATGGCC3130.1123349519615549No Hit
TCTAGTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTT3130.1123349519615549No Hit
TCTAGTGTTGAGATCGTTTCGGCCCCAAGACCTCTAGTCATTCGCTTTAC3110.11161715674135327No Hit
TCTAGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTC3080.11054046391105081No Hit
TCTAGTCCAAGATCTGCACCCGCGGCGGCTCCACCCGGGCCCACGCCCTG3040.10910487347064757No Hit
TCTAGTATTAAATCAGTTATGGTCCCTTTGATCGCTCCCATCCGTTACTT3010.10802818064034511No Hit
TCTAGTGGTAAAGCGAATGACTAGAGGTCTTGGGGCCGAAACGATCTCAA3000.10766928303024431No Hit
TCTAGTCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTT3000.10766928303024431No Hit
TCTAGTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTT2990.10731038542014348No Hit
TCTAGTGGTAATTCTAGAGCTAATACATGCAAACGAGCGCTGACCTCCGG2970.10659259019994187No Hit
TCTAGTAAGAAGTTGGCCGCCGACCGCTCGGGGCCCCGTAACTATTTAGC2960.10623369258984103No Hit
TCTAGTCAAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCG2920.10479810214943779No Hit
TCTAGTGTTATTTTTCGTCACTACCTCCCCGCGTCAGGAGTGGGTAATTT2900.10408030692923617No Hit
TCTAGTCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCA2890.10372140931913533No Hit
TCTAGTGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTTTC2890.10372140931913533No Hit
TCTAGTAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGT2880.10336251170903453No Hit
TCTAGTGTAACTTTTCTGACACCTCCTGCTTGAAACCCAAAAAGCCAGAA2860.10264471648883292No Hit
TCTAGTGCCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTG2840.10192692126863127No Hit
TCTAGTGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGG2840.10192692126863127No Hit
TCTAGTGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCG2830.10156802365853046No Hit
TCTAGTAAGGTTTTAGCACCGAGTTCCACACGAACGTGGGGTGCGTCGAC2790.10013243321812719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTG119650.0474.022281
CTAGTGT41200.0469.895752
CTAGTGC32750.0467.800262
TAGTGCG7100.0464.635533
TAGTCGC8450.0463.958163
CTAGTCG26900.0457.58172
CTAGTCC33250.0455.73392
TAGTGTT14750.0452.17223
TAGTGCC11950.0452.09763
TAGTGTC12600.0449.644683
TAGTCCC12300.0448.95063
TAGTATC4100.0448.950563
CTAGTAT12100.0448.388242
CTAGTGA16450.0443.146822
TAGTGCA5900.0441.637733
TAGTGCT9050.0441.12293
TAGTCTC13500.0439.146643
TAGTGTG9650.0438.46773
TAGTGGC11800.0433.534273
TAGTCCG11150.0433.080023
Produced by FastQC (version 0.11.4)