All statistics are based on contigs of size >= 500 bp, unless otherwise noted (e.g., "# contigs (>= 0 bp)" and "Total length (>= 0 bp)" include all contigs). Suggestion: assembly arowana_transcripts.fasta.transdecoder.cds contains continuous fragments of N's of length >= 10 bp. You may consider rerunning QUAST using --scaffolds (-s) option! Assembly arowana_transcripts.fasta.transdecoder.cds cds-no_redundancy # contigs (>= 0 bp) 47765 40089 # contigs (>= 1000 bp) 31694 14122 # contigs (>= 5000 bp) 2401 198 # contigs (>= 10000 bp) 296 9 # contigs (>= 25000 bp) 6 0 # contigs (>= 50000 bp) 2 0 Total length (>= 0 bp) 88693317 40348176 Total length (>= 1000 bp) 78588546 26081310 Total length (>= 5000 bp) 17845038 1257993 Total length (>= 10000 bp) 4113786 109917 Total length (>= 25000 bp) 287265 0 Total length (>= 50000 bp) 153492 0 # contigs 42098 27136 Largest contig 88050 14787 Total length 86541075 35409537 GC (%) 54.72 54.87 N50 2583 1524 N75 1521 975 L50 9748 7321 L75 20692 14604 # N's per 100 kbp 11.26 0.00