All statistics are based on contigs of size >= 500 bp, unless otherwise noted (e.g., "# contigs (>= 0 bp)" and "Total length (>= 0 bp)" include all contigs). Suggestion: assembly arowana_transcripts.fasta.transdecoder.cds contains continuous fragments of N's of length >= 10 bp. You may consider rerunning QUAST using --scaffolds (-s) option! Assembly # contigs (>= 0 bp) # contigs (>= 1000 bp) # contigs (>= 5000 bp) # contigs (>= 10000 bp) # contigs (>= 25000 bp) # contigs (>= 50000 bp) Total length (>= 0 bp) Total length (>= 1000 bp) Total length (>= 5000 bp) Total length (>= 10000 bp) Total length (>= 25000 bp) Total length (>= 50000 bp) # contigs Largest contig Total length GC (%) N50 N75 L50 L75 # N's per 100 kbp arowana_transcripts.fasta.transdecoder.cds 47765 31694 2401 296 6 2 88693317 78588546 17845038 4113786 287265 153492 42098 88050 86541075 54.72 2583 1521 9748 20692 11.26 cds-no_redundancy 40089 14122 198 9 0 0 40348176 26081310 1257993 109917 0 0 27136 14787 35409537 54.87 1524 975 7321 14604 0.00