FastQCFastQC Report
Qua 4 jul 2018
reads_R1.t.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamereads_R1.t.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138094780
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70316155.091876028912896No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3241290.23471488205419494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT276650.023.574591
CGGTATT305550.020.8424662
TTGGGCG373100.020.72531749
GCCACAT1138150.019.0070041
TATTGGG458950.017.031447
TTATTGG483900.016.40125346
ATTGGGC482300.016.21564348
CCACATA640900.015.7273052
TCTCGAC363400.015.50272445
CACATAT595850.015.4448543
GCCATAT321500.014.9385561
GGGCAAT450850.014.9197571
GCCAGAT797350.014.2884261
TGGGCGA559600.014.20762250
CGAGGCT558650.014.08308254
TACGTAT125400.013.95545834
GCCCATT485800.013.9165981
GCGAGGC585650.013.66379553
TTGTCGG459000.013.5461728
GCCGTAT124800.013.5293691
Produced by FastQC (version 0.11.4)