FastQCFastQC Report
Qua 4 jul 2018
reads_R2.t.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamereads_R2.t.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138094780
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61265524.436483406541507No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3107640.22503674650120736No Hit
GGGGGGGGGGGGGGGGGGGG2561740.18550592571276045No Hit
GGGGGGGGGGGGGGGGGGGGG2323140.1682279373630198No Hit
GGGGGGGGGGGGGGGGGGGGGG2034970.1473603853816922No Hit
GGGGGGGGGGGGGGGGGGGGGGG1988460.14399240869205918No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1886240.136590246206265No Hit
GGGGGGGGGGGGGGGGGGGGGGGG1844910.13359737420922066No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1735670.12568686520953218No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG1689750.12236161279955696No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGG1528710.11070005687398177No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1524520.11039664207437819No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGG1457110.10551521208839319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGCG347750.020.73398649
GCGGTAT235800.019.7582171
GCCACAT1038250.017.4021471
TATTGGG433300.016.65334347
CGGTATT276300.016.6036512
TTATTGG451250.015.9336846
ATTGGGC455150.015.88806648
TGGGCGA509300.014.57071250
CGAGGCT515950.014.30705854
CACATAT563800.013.8494683
TCTCGAC331250.013.8048745
CCACATA641300.013.7603832
GCGAGGC557700.013.45178853
GCCAGAT748350.013.2669881
GCCATAT300150.013.2073881
ATATTTC566600.012.5321936
TACGTAT112250.012.50486734
GGGCAAT409950.012.3468121
GCCCATT442100.012.2053471
GGAAATA608300.012.032538
Produced by FastQC (version 0.11.4)