running histogram estimation debug (read file is a list: False read files list: ['../reads_R1.t.fastq'] Setting maximum kmer length to: 76 bp computing histograms (from k=21 to k=71): 31 41 61 71 21 51 ntCard wall-clock time over all k values: 1136 seconds fitting model to histograms to estimate best k could not fit histograms-k61.histo could not fit histograms-k71.histo could not fit histograms-k41.histo could not fit histograms-k51.histo could not fit histograms-k31.histo could not fit histograms-k21.histo could not predict a best k value