FastQCFastQC Report
Thu 13 Feb 2020
SRR594393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594393_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87264604
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA268350.037.2648931
GACCGAT250450.036.61558532
CGAGACC275700.036.03066329
AGACCGA277600.035.13430431
ACCGATA121700.035.1005433
TATGCCG173600.035.0469243
AATGCCG306250.033.29838624
ATGCCGA306000.033.260925
GAGACCG305700.032.73223530
GAGCGGT317150.032.6913159
AGCGGTT316800.032.6857710
CCGAGAC312050.031.83353428
GCGGTTC327850.031.48346111
TGCCGAG327650.031.05641626
CCGATAT132450.030.92308834
GCCGAGA330300.030.74727627
GATATCG131600.030.53780236
AAGAGCG352150.030.4323487
TCGTATG230150.030.38328440
ACCGATC134700.030.30857333
Produced by FastQC (version 0.11.4)