FastQCFastQC Report
Thu 13 Feb 2020
SRR594393_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594393_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87264604
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTTA393200.019.3971251
GATTTAG406100.018.53942
TAGGGCG135900.014.42548517
CGGAATT131500.013.7549752
TTAGCTC559600.013.6393855
ATTTAGC561500.013.5366393
TAGCTCA571450.013.4317586
TAGAGCG258350.013.16033217
CCGGAAT147950.013.1453931
CGGCCCG137050.013.0302248
TCACCCG138850.012.9867593
GCGCTTG310700.012.78258921
CGCTTGC306050.012.4311522
AGTGGTA619800.012.16506412
ACCCGGC144750.012.0803565
GTGGTAG644300.011.65520113
GCAAGCG381100.011.30939332
TTTAGCT698350.011.0151434
CCGGACA170500.010.67524412
AGCGCAA393500.010.66745935
Produced by FastQC (version 0.11.4)