FastQCFastQC Report
Thu 13 Feb 2020
SRR594394_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594394_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101816491
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA215250.037.0324481
CGAGACC228000.033.26797529
ACCGATA107750.031.83146733
AGACCGA242750.030.95763831
TATGCCG153300.030.95124443
AGCGGTT259950.030.6148310
GAGCGGT262400.030.5460199
ATGCCGA256850.030.1281625
GACCGAT237900.030.02268232
GAGACCG266200.028.749230
GATATCG115050.028.17343736
TCGTATG194800.027.66498640
CCGAGAC276550.027.55410828
CCGATAT120200.027.44047534
AATGCCG284100.027.3926424
TGCCGAG287800.026.86533426
GCCGAGA296250.026.23220427
TATCGTA103350.026.1675738
ACCGATC123450.025.6351433
GCGGTTC317200.025.13765511
Produced by FastQC (version 0.11.4)