FastQCFastQC Report
Thu 13 Feb 2020
SRR594394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101816491
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG190900.017.2022573
ACCCGGC212350.015.4235845
CGGAATT187500.015.3508062
CCGGAAT225100.014.619231
CGGCCCG224700.014.3950628
CACGGAC241050.013.21714817
CCCGGAC246950.012.98956911
CCGGACA249150.012.90138112
CACCCGG253000.012.8931224
CGACGAC146450.012.0855481
CACGATA231200.012.03887542
ACGATAC231550.011.94471743
ACGGACA273250.011.87688818
CGATACA238450.011.61751544
ATAGCTC264950.011.30194539
CGGACAC285650.011.15308213
GCCCGGA296800.010.98629410
CGGACAG301800.010.753345519
TGATAGC289500.010.62451637
ATCGTGC268200.010.6075633
Produced by FastQC (version 0.11.4)