FastQCFastQC Report
Thu 13 Feb 2020
SRR594395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35175982
Sequences flagged as poor quality0
Sequence length36
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG1200830.34137781853538585Illumina Paired End PCR Primer 2 (100% over 36bp)
GGCTTTTGCTCATGTGTCATCTAGGGTGAAGCCTGA785050.22317784902209695No Hit
CTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAA727050.20668932568819257No Hit
CTTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAA628500.17867305026480854No Hit
GTTCGATTCCTTCCTTTCTTATTTTACTTTTACATA623050.17712369764119165No Hit
TTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAAA604310.17179619889503014No Hit
CTACTACATAGTATGTATCGTGAAGCACGATGTCAA586020.16659662834714892No Hit
GGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGCCTGT545500.15507740480422125No Hit
GCAGCCATGAAGTCATTCTAAATTTGTTGAAGCATA530310.1507591174000487No Hit
CTCGAATGTGTGATATGGTGGAGGGCAGCCATGAAG481690.13693718628807577No Hit
TTCGATTCCTTCCTTTCTTATTTTACTTTTACATAG479920.13643400204150663No Hit
TGAAGCCTGAAAATAATGGGAATCAGTGAACAAATC435320.12375489616750429No Hit
CTACATAGTATGTATCGTGAAGCACGATGTCAAGGG435150.12370656773704285No Hit
GCGGTCTGTTAGTAGTATAGTAATGCCTGCGGCTAG431940.12279401325597677No Hit
GTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTAT425710.12102291842200738No Hit
GTGAATACGTAGGCTTGAATTAATGCTACTGCAAAT423980.12053110557084092No Hit
CTTGATTTATGTGGTTTCGTTTACCTTCTATAAGGC414380.11780197067419468No Hit
TGCAAGTCAGCTAAATACTTTGACACCGGTAGGAAT398350.1132448839665656No Hit
GTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGC384380.10927342412217517No Hit
GCGGAATATTAGGCTTCGTTGCTTTGAGGTATGAAG384330.10925920987792181No Hit
GTAAAGTCCTAGTATAATGGTAATTAGTAGGGCTTG383590.10904883906297201No Hit
CAGCCATGAAGTCATTCTAAATTTGTTGAAGCATAC374290.10640498963184597No Hit
GCAAACAATAAGGAATGTTGATCCAATAATTACATG355290.10100357681556693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA138000.028.6552771
ATCGGAA142950.027.6950652
CGAGACC136000.027.57268529
GAGCGGT143750.027.1709949
GAGACCG138500.027.04249430
ATGCCGA143400.026.8498425
AGCGGTT146050.026.65067510
GCCGAGA145350.026.37652827
GCGGTTC148600.026.19334611
AGAGCGG154800.025.6033488
AATGCCG151250.025.5646724
CCGAGAC147800.025.452528
AAGAGCG169600.024.1638647
GAATGCC161200.024.0684223
CGGTTCA162800.023.9455212
TGCCGAG165400.023.26048326
CGGGCTA25600.022.2521361
CGTAACG22800.021.7756530
CGGAAGA189550.020.900684
CTCGAAT85250.019.3924261
Produced by FastQC (version 0.11.4)