FastQCFastQC Report
Thu 13 Feb 2020
SRR594395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35175982
Sequences flagged as poor quality0
Sequence length36
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAATTGTTTTATCCAACTCATCCCTTGACATCG1541040.4380943792841377No Hit
CGGAATTGTTTTATCCAACTCATCCCTTGACATCGT726300.20647611202439212No Hit
GTTTGCTATCATAGCAGGATTTGTTCACTGATTCCC599320.17037761731854423No Hit
CGCAATTCCTACCGGTGTCAAAGTATTTAGCTGACT552790.15714984161636197No Hit
CCGGCTTACAGCTAACATTACTGCAGGACACTTATT545280.15501486212950644No Hit
AGTTAATCCAAGTCCATGACCATTAACTGGAGCCTT431220.12258932813872829No Hit
TGCTATCATAGCAGGATTTGTTCACTGATTCCCATT391960.11142830355098543No Hit
CGAACATGAACCCTAATAATTGTTTCCCTAATCATA361480.10276330025413363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT331750.015.7495751
TCACCCG38100.015.6706863
CGCAATT116750.015.5147431
ACCCGGC40000.014.6390115
TACCGGT124800.014.29963510
CACCCGG43500.013.8539614
TTAATCG20650.013.72155429
ACCGGTG131650.013.634500511
CGGAATT371300.013.5613462
CCTACCG133150.013.5377148
CTTGACA373450.013.40776427
ATCCCTT376850.013.30759823
CTACCGG135950.013.2802749
TCCCTTG376700.013.26247924
CCTTGAC394300.013.17471126
CCCTTGA383250.013.106832525
GAATTGT395450.012.8946964
GGAATTG402950.012.8090993
CGAACAT88750.012.7983381
TGTTTTA395400.012.7813088
Produced by FastQC (version 0.11.4)