FastQCFastQC Report
Thu 13 Feb 2020
SRR594396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119274786
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGCTCATGTGTCATCTAGGGTGAAGCCTGAAAATAATGGGAATCAGT1421910.11921295754829524No Hit
GTGAAGTCCATGATCTGCTCAGAGTCAGTGCTTAAATCATCATCTGTATA1378920.11560867524843012No Hit
TTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTTGGTTCCTCGAA1248160.10464575471969408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA414550.038.355631
TATGCCG303900.037.17679643
GACCGAT400650.036.69795632
AGACCGA421250.036.3243731
CGAGACC425700.036.303829
ACCGATA179200.036.1982633
GAGCGGT462850.034.795679
AGCGGTT472800.033.99362610
ATGCCGA464550.033.73863625
GCCGAGA465550.033.62349327
GAGACCG462200.033.5161630
ACCGATC221650.033.1273533
GATATCG186400.032.98008336
CCGATAT193350.032.69413434
AATGCCG496350.031.61690124
CGATATC200100.031.36013235
CCGAGAC495350.031.30676828
TGCCGAG506300.030.93043126
GCGGTTC527050.030.4987611
TCGTATG395750.030.36864940
Produced by FastQC (version 0.11.4)