FastQCFastQC Report
Thu 13 Feb 2020
SRR594396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119274786
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAATTGTTTTATCCAACTCATCCCTTGACATCGTGCTTCACGATACA1495630.12539364354843613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT146600.020.4413471
CCGGAAT406700.019.7661911
TTAATCG112000.018.74062229
CGGCTTA224050.018.170992
CGGAATT440000.016.929182
GTTAATC216900.014.3541172
CGAACAT157050.013.865761
GAATTGT594700.013.5812034
ATAGGCC165450.013.06920920
ATCGTGC541450.013.05857933
GGAATTG693250.013.0191143
TCACCCG182900.013.0138383
CGCGAAT44200.012.9021721
CACGATA548200.012.8184142
CCGGCTT523350.012.7225711
CAATCTA215800.012.59134
GACAATC251300.012.4154932
ACGATAC573800.012.28103643
CGGCTAT230050.012.1744751
TCGTGCT590800.012.12161234
Produced by FastQC (version 0.11.4)