FastQCFastQC Report
Thu 13 Feb 2020
SRR594397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116292478
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCC3908610.33610170384364846No Hit
GGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTC2614880.22485375193398147No Hit
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT2516150.2163639508997306No Hit
CTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGA2462570.2117566021767977No Hit
CTTTTCTACATTAAAGTTCCTTCCCGTAGAACTAGCTTCTTCTGCATGGA2244820.1930322612955242No Hit
GCCAGGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTT2143140.1842887895122503No Hit
CTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCAC2089170.17964790465639574No Hit
GCCATTATCTTCTATCTTTTCTCTTTTGTCAGAGGCCAGGATAATAGTAT1901330.16349552719996216No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT1833320.15764734155892696No Hit
TGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAA1625990.13981901735725333No Hit
CTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAAGGA1465560.1260236281146232No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT1438560.1237018958354297No Hit
GAAAAAGTCTAAAGTTGCCATTATCTTCTATCTTTTCTCTTTTGTCAGAG1369820.11779093743277187No Hit
GAAAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTATCCTAGGTAACAT1173710.10092742197822975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA243150.037.390841
ACCGATA107100.036.12390533
AGACCGA241250.035.5285231
CGAGACC250350.034.75395629
TATGCCG170000.034.42807843
GAGACCG258050.033.75948330
GAGCGGT270500.033.419929
ACCGATC110600.033.2704933
AATGCCG277500.032.00379624
GACCGAT260000.031.42675432
CCGAGAC291500.029.8704128
AGCGGTT307250.029.39383310
ATGCCGA303300.029.16539225
CCGATAT132450.028.51258934
CGGAAGA340850.027.1521644
TATCGTA117750.027.02871338
AGAGCGG344900.026.9054878
TGCCGAG331200.026.65535426
GCGGTTC340200.026.52101511
GATATCG143050.025.87707336
Produced by FastQC (version 0.11.4)