FastQCFastQC Report
Thu 13 Feb 2020
SRR594397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116292478
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTAC2655840.22837590579160247No Hit
CACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCT2439190.20974615400318497No Hit
CTGTGATCTGCATTCCATCCTGTCTCACTGAGAAGTCCAATTCCAGTCTA2110210.1814571360324784No Hit
CTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACCTTCCAGC1908100.16407768007144882No Hit
GCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAG1706660.146755837466977No Hit
CGAAAAGGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAAC1705810.14668274589522462No Hit
CGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCT1589520.13668295897865382No Hit
GTTTGCACAACTATGTGAGAAGCATGGAATCCTTAGAGAAAATATCATTG1340390.11526024924845095No Hit
AGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTG1332500.11458178748241998No Hit
GGAATCCTTAGAGAAAATATCATTGACCTATCCAATGCCAATCGCTGCCT1311760.11279835313166171No Hit
GACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATG1294100.11127976824090033No Hit
TGAAGATGCTGCTGCTGCTGTGTTTGGGACTGACCCTAGTCTGTGTCCAT1252550.10770688023347477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC460900.029.2315041
ACGATCA598700.022.319692
ACCCGGC234600.021.4467265
CGATCAC791300.018.1966381
CGGCCCG294950.017.1479978
CCGGACA309050.016.18767212
ACGGACA314550.016.00953718
CACCCGG323950.015.7962714
CCCGGAC327400.015.4483811
CGGACAG332150.014.98238719
GGACACG336300.014.78441714
CACGGAC338500.014.74682317
TCACAAG1009500.014.4465634
TCACCCG368600.014.1991323
ATAGCTC330800.014.13240539
CGGACAC356150.014.022181513
CCCGGCC377300.014.0058366
TGATAGC354150.013.5919837
TTGATAG365650.013.44126936
ATTGATA360050.013.38147535
Produced by FastQC (version 0.11.4)