FastQCFastQC Report
Thu 13 Feb 2020
SRR594398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34050626
Sequences flagged as poor quality0
Sequence length36
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG626810.18408178457570795Illumina Paired End PCR Primer 2 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA80100.026.597981
ATGCCGA85250.024.28057125
ATCGGAA88300.024.0756322
GAGCGGT88450.024.027259
CGAGACC85700.023.8917729
AATGCCG88000.023.75986924
AGCGGTT89500.023.57779110
GAGACCG87750.023.36783630
CCGAGAC90300.022.7243628
TGCCGAG92200.022.49934626
GCGGTTC94950.022.05070511
GCCGAGA95650.021.71962527
AGAGCGG102300.020.8601468
AAGAGCG109350.020.597527
CGGTTCA104200.020.12201712
TCGTTCG26150.019.96185128
TCGGAAG111250.019.167413
CGGAAGA120800.017.987274
CGCGTAA35950.016.9741441
GAATGCC128500.016.91293523
Produced by FastQC (version 0.11.4)