FastQCFastQC Report
Thu 13 Feb 2020
SRR594398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR594398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34050626
Sequences flagged as poor quality0
Sequence length36
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG50350.012.6616483
ACCCGGC49550.012.5137425
CCGGAAT43750.011.3783031
CGGCCCG53450.011.2520128
CACCCGG58950.010.5775724
CGGAATT40900.010.2940612
CCGGACA60200.09.76545412
CGCAATT13350.09.6882371
CCCGGAC61650.09.68118711
ACGGACA62350.09.47195818
CACGGAC64850.09.06035617
CGACAAT18600.08.5707821
ACATCTA94250.08.5608064
CGCGCTA25300.08.5584272
GACACGG69550.08.49178415
CGGGAAT24550.08.4538621
CGGACAG72150.08.41397719
CGGACAC70650.08.36376213
ACACGGA90800.08.00622316
GCCCGGA77750.07.947530710
Produced by FastQC (version 0.11.4)