FastQCFastQC Report
Mon 19 Aug 2019
polipo.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamepolipo.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451057
Sequences flagged as poor quality0
Sequence length72
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA34440.23734422562311475TruSeq Adapter, Index 5 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA23950.16505209650620203TruSeq Adapter, Index 5 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG5650.052.68058840
GTGGATC9600.038.70508638
AGTGGAT9800.038.6734937
GATCTCG10500.037.93617641
GTGAATC6300.037.74667437
GGATCTC10850.036.6957640
GAATCTC7050.036.3869639
TATCGCG350.00298378533.5354822
AGATCGG9850.033.191651
TGGATCT11900.032.4916839
GCGCTAA550.00707782628.10651842
TCGTATG15900.027.24864845
CGCGCAT557.795528E-427.0196481
CCGTCTT16150.026.7416552
TGAATCT9650.026.56813438
CGTATGC16200.026.5088746
GCCGTCT16300.026.4919351
CTCGTAT16250.026.16944
ATGCCGT16650.026.1686949
AGTGAAT8000.025.96243936
Produced by FastQC (version 0.11.4)